The first successful de novo design of a homo-trimeric protein that binds a C3 symmetric small molecule larger than a metal ion is an advance for computational protein design.
Deep sequencing of transcriptomes from closely related taxa shows that the whole genome can become subject to evolutionary testing within a short time frame via de novo evolution of genes.
A novel lncRNA (Ephemeron) is connected to known post-transcriptional and epigenetic regulators as part of an integrated machinery, which controls the timely exit from the naïve state of mouse embryonic stem cells.
Single-cell RNA-sequencing and germline substitutions provide novel insights into how testis is a hotspot for evolutionary innovation of genes, expression, and mutation at the single-cell level.
Many vertebrates are able to synthesize a sunscreen compound de novo and the pathway involved can be used for heterologous production of the compound in yeast.
Direct live-cell imaging of human cells, combined with RNA-seq, qPCR and in vitro reconstitution essays, reveal that mitotic progression, arrest, exit or death is independent of de novo transcription.
Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.
Canonical centriole duplication is much less prone to errors than de novo synthesis of centrioles, but neither depends on self-oligomerization of the centriole protein SAS-6.
