Browse the search results

Page 2 of 153
    1. Structural Biology and Molecular Biophysics

    Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

    Rhys M Adams et al.
    The absolute affinities of thousands of variant antibodies are measured in parallel using a combination of cell sorting and high-throughput DNA sequencing.
    1. Computational and Systems Biology

    Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting

    David Mavor et al.
    Chemical perturbation-dependent deep mutational scanning data collected by a lab-based interdisciplinary graduate class resolves a paradox between the high evolution conservation and the high mutational tolerance of the protein ubiquitin.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Uncovering the basis of protein-protein interaction specificity with a combinatorially complete library

    Thuy-Lan V Lite et al.
    A generalizable approach to understanding the logic of molecular recognition reveals the contributions of individual residues to the specificity of protein-protein interactions.
    1. Structural Biology and Molecular Biophysics

    Mutational scanning reveals the determinants of protein insertion and association energetics in the plasma membrane

    Assaf Elazar et al.
    Deep sequencing analysis quantifies the hydrophobicity of the plasma membrane and provides restraints for structure modeling of receptor homodimers to atomic precision.
    1. Developmental Biology
    2. Evolutionary Biology

    An integrative genomic analysis of the Longshanks selection experiment for longer limbs in mice

    João PL Castro et al.
    Genome sequencing of mice selected for longer limbs reveals that rapid selection response is due to both discrete loci and polygenic adaptation.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Alzheimer’s Disease: Exploring the origins of nucleation

    Katarzyna Marta Zoltowska, Lucía Chávez-Gutiérrez
    An approach called deep mutational scanning is improving our understanding of amyloid beta aggregation.
    Insight
    Available as:
    • HTML
    • PDF
    1. Immunology and Inflammation
    2. Physics of Living Systems

    Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling

    Doug K Tischer, Orion David Weiner
    Direct control of ligand binding half-life with light shows that lifetime strongly affects T cell signaling.
    1. Neuroscience

    Drep-2 is a novel synaptic protein important for learning and memory

    Till F M Andlauer et al.
    Drep-2 is the first representative of the evolutionary conserved CIDE-N protein family found at synapses and is required for associative learning by functionally intersecting with metabotropic signaling.
    1. Neuroscience

    Acute cerebellar knockdown of Sgce reproduces salient features of myoclonus-dystonia (DYT11) in mice

    Samantha Washburn et al.
    Acute knock down of Sgce that is mutated in Myoclonus Dystonia (DYT11), in the cerebellum of mice, leads to dystonia and myoclonus-like motor signs that like patients improve with alcohol.
    1. Microbiology and Infectious Disease

    Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

    Karthik Hullahalli et al.
    Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.