A series of genetically-encoded fluorescent reporters that allow imaging of neuropeptide release in vivo, with sub-second temporal and nerve terminal-level spatial resolution are developed.
Clarinet, a novel C. elegans active zone protein with homology to vertebrate Piccolo and Rim, uses its different isoforms for diverse functions, including synaptic vesicle clustering, vesicle release and synaptogenesis.
Spontaneous osmoregulation in giant phospholipid vesicles, which is characterized by oscillations in vesicle size and membrane tension, couples to the compositional degrees of freedom at the membrane surface to produce oscillations in domain texture and induce isothermal phase change.
An unbiased model for the self-organisation of the Golgi apparatus displays either anterograde vesicular transport or cisternal maturation depending on ratios of budding, fusion and biochemical conversion rates.