For the first time, the spectrum of genes and pathways interacting with alternative DNA structures called G-quadruplexes in humans is revealed highlighting new possibilities for the pursuit of cancer therapies.
Obligate intracellular Chlamydia secrete a deubiquitinating enzyme (Cdu1) into the membrane of the Chlamydia-containing vacuole to deubiquitinate selected host proteins and support the survival of the bacteria during genital infection.
Ubiquitination close to the active site of RNAPII occurs in response to RNA processing events and is linked to transcriptional pausing, which is released following Bre5-Ubp3 mediated deubiquitination associated with the nascent transcript.
An atomic model of the 3744-residue Tra1 protein reveals multiple transcription activator binding sites, its integration within the SAGA chromatin coactivator complex, and a striking similarity to DNA-repair factor DNA-PKcs.
Engineered E3 ubiquitin ligases are utilized to elucidate mechanisms underlying ubiquitin regulation of membrane proteins, and to achieve robust post-translational functional knockdown of ion channels.