ATP consumption enables chaperones to exploit the different kinetic properties of their conformational states to exhibit a non-equilibrium affinity for their substrates that is orders of magnitude higher than its equilibrium value.

The absolute affinities of thousands of variant antibodies are measured in parallel using a combination of cell sorting and high-throughput DNA sequencing.

The antibiotic viomycin induces errors during initial codon selection by the ribosome by locking the monitoring bases in their active conformation.

Molecular details of how the iron–sulfur cluster cofactor of a bacterial global iron regulatory protein simultaneously senses iron and O₂ are revealed by mass spectrometry and spectroscopy.

Detection of unbinding transitional states in the charybdotoxin first-order dissociation from a Kv-channel reveals that the bound neurotoxin wobbles, suggesting diverse intermediates and dissociation pathways in this protein–protein interaction.

Profilin helps to maintain formin at the growing barbed end of an actin filament, while piconewton mechanical tension drastically enhances formin dissociation from the barbed end.

Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.

The budding yeast transcription factors Reb1 and Cbf1 function as pioneer factors by slowly dissociating from nucleosomes, allowing them to target and unwrap nucleosomes efficiently to regulate transcription.

Gating currents analysis at different Ca²⁺ concentrations of BK channels reveals a strong allosteric coupling between Ca²⁺- and voltage-sensing modules via equivalent contributions exerted by the RCK1 and RCK2 Ca²⁺-sites.

A combined experimental and computational approach was developed to understand lateral interactions between membrane-bound proteins and used to quantify the contributions of specific and non-specific interactions to cadherin cis-binding kinetics.