Pulse electron paramagnetic resonance on intact Escherichia coli reveals a substrate-induced structural transition in BtuB, the vitamin B12 transporter, which is not seen in reconstituted membranes and indicates a mechanism of transport.
A quantitative understanding of molecular tension sensor function enables the production of unique sensors with desired mechanical properties as well as the ability to distinguish between protein force and protein deformation in mechanosensitive processes.
NHE1-CaM complexes of multiple stoichiometries regulate cellular Ca2+-dependent NHE1 activity and can contribute to NHE1 dimerization, the latter shown by the NMR structure of CaM linking two NHE1 cytosolic tails.
Structural and functional analysis of a new class of low-molecular-weight antibody fragments, derived from bovine immunoglobulins, reveals their therapeutic potential against C5, a target for refractory inflammatory diseases.
A peptide derived from BK and JC polyomavirus protein VP2/3 inhibits viral infection by targeting a binding site in the pore of polyomavirus VP1 pentamers, enabling future VP1-targeted therapeutic strategies.
Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.