5,936 results found
    1. Cell Biology
    2. Developmental Biology

    An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

    Pooran Singh Dewari et al.
    Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
    1. Genetics and Genomics

    An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

    David Li-Kroeger et al.
    New technology for manipulating genes in Drosophila facilitates gene tagging and precise modification of virtually any gene.
    1. Neuroscience

    Group III metabotropic glutamate receptors gate long-term potentiation and synaptic tagging/capture in rat hippocampal area CA2

    Ananya Dasgupta et al.
    Group III metabotropic glutamate receptor inhibition facilitates synaptic plasticity in the plasticity-resistant synapses of hippocampal area CA2, a brain region critical for social memory.
    1. Cell Biology

    Male meiotic spindle features that efficiently segregate paired and lagging chromosomes

    Gunar Fabig et al.
    Chromosome segregation in male spermatocytes exhibits anaphase A without shortening of autosome-associated microtubules and partitioning of an unpaired X chromosome that is initiated by an imbalance of attached kinetochore microtubules.
    1. Developmental Biology
    2. Neuroscience

    A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

    Sonal Nagarkar-Jaiswal et al.
    A conditional gene inactivation system that permits the tracking of protein expression, and creates fluorescently-labelled mutant cells in both developing and adult Drosophila tissues.
    1. Cell Biology
    2. Neuroscience

    A genetic toolkit for tagging intronic MiMIC containing genes

    Sonal Nagarkar-Jaiswal et al.
    Building on previous work (Nagarkar-Jaiswal et al., 2015), we report an elegant genetic strategy for endogenous tagging of MiMIC containing genes.
    1. Biochemistry and Chemical Biology

    Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation

    Roxana E Georgescu et al.
    The different polymerases at eukaryotic replication forks achieve their asymmetric placement on the leading and lagging strands through exclusion processes that prevent their function on the 'wrong' strand.
    1. Developmental Biology

    Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    Emilie Auxerre-Plantié et al.
    Drosophila DM1 models suggest that elevated cardiac expression of straightjacket/α2δ3, a regulatory subunit of voltage-gated calcium channel, contributes to cardiac conduction defects in DM1.
    1. Neuroscience

    Neural markers of predictive coding under perceptual uncertainty revealed with Hierarchical Frequency Tagging

    Noam Gordon et al.
    The novel neural marker for the integration of top-down predictions and bottom-up signals in perception elucidates uncertainty in perceptual inference and provides evidence for the predictive coding account of perception.
    1. Genetics and Genomics

    One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

    Wenyuan Li et al.
    Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.

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