Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
Chromosome segregation in male spermatocytes exhibits anaphase A without shortening of autosome-associated microtubules and partitioning of an unpaired X chromosome that is initiated by an imbalance of attached kinetochore microtubules.
The different polymerases at eukaryotic replication forks achieve their asymmetric placement on the leading and lagging strands through exclusion processes that prevent their function on the 'wrong' strand.
The novel neural marker for the integration of top-down predictions and bottom-up signals in perception elucidates uncertainty in perceptual inference and provides evidence for the predictive coding account of perception.
Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.