By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.

T cells, neutrophils, and protista Dictyostelium undergo amoeboid durotaxis which involves soft end polarized NMIIA.

Unconventional secretion of a plasma membrane purine transporter via Golgi-bypass is established at an early ER-associated secretory compartment revealing that specific cargoes define alternative trafficking routes.

Experiments on mice show that TMC7, a transmembrane channel-like protein, localizes to the cis-Golgi region in spermatids and that this is essential for acrosome biogenesis and male fertility.