The first genomic view of beetle luciferase evolution indicates evolutionary independence of luciferase between fireflies and click-beetles, and provide valuable datasets which will accelerate the discovery of new biotechnological tools.
Spenser S Smith, Daniel Chu ... Richard A Schneider
Multiple levels of gene regulation in the TGFβ signaling pathway mediate the expression of Mmp13, establish species-specific domains of bone resorption, and likely generate evolutionary variation in jaw length.
Repulsive guidance cues enhance a dual-functioning axonal trafficking facilitator, allowing navigating axons to ensure proper pathfinding by coordinating positive extension and negative steering during nervous system development.
Alfonso Bolado-Carrancio, Oleksii S Rukhlenko ... Boris N Kholodenko
Different RhoA and Rac1 dynamics at the cell front and rear are coordinated through periodic GTPase waves, which define the minimal autonomous biochemical machinery necessary and sufficient for cell migration.
Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.
Genetic experiments in zebrafish identify neuropeptide VF (NPVF) signaling and NPVF-expressing neurons as a novel vertebrate hypothalamic sleep-promoting system that is functionally conserved with invertebrates.
The small, highly conserved transmembrane protein disrupted in zebrafish dreammist mutants regulates the activity of the sodium-potassium pump, leading to changes in sleep behaviour.
