Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.
One-step Isolation and Lysis (OIL) PCR offers a robust, versatile, accessible, and high-throughput method for linking mobile DNA with bacterial hosts in natural microbial communities.
Resolving parallel and concurrent cytokine secretion is essential to studying polyfunctional cytokine-secreting cells, and to differentiating immune response granularity from unwinding the complexity and heterogeneity of cytokine responses.
Under fluid shear, living cells deform elliptically, align in flow direction, and rotate, from which their frequency-dependent viscoelastic properties can be inferred.
Amichai Baichman-Kass, Tingting Song, Jonathan Friedman
High-throughput measurements of simplified bacterial communities find that when multiple species jointly inhibit a focal species of interest, their individual effects do not add up, but are dominated by the strongest single-species effect.
The regulatory subunit of protein kinase A (PKA) can both activate and suppress the PKA activity at the plasma membrane, with its graded membrane localization capable of inducing a switch in cell migration.
Pascal D Odermatt, Teemu P Miettinen ... Fred Chang
The density of the cytoplasm in S. pombe varies in time and space during the cell cycle through modulation of tip growth rate coupled with constant mass growth rate.
