The Bi-Genomic Mitochondrial-Split-GFP, where both fragments of the Split-GFP are expressed by separated translation machineries, shuts off cytosolic fluorescence of dual-localized proteins, allowing visualization of their mitochondrial echoforms.

Intracellular protein gradients, which are formed by the combined action of cytoplasmic diffusion and cortical transport toward the cell pole, are solely determined by cell shape.

The protein-lipid modification palmitoylation targets the kinase LIMK1 to dendritic spines to ensure precise control of the size of individual spines in response to synaptic activation.

Genetically-encoded platforms direct kinase inhibitor drugs to organelles to reveal new dimensions of local kinase signaling.

The dual function of an ancient prokaryotic enzyme, which is linked to specific metabolite signals, may have been the evolutionary driving force behind its dual localization in eukaryotes.

An under-studied microtubule-associated protein is found to regulate axon growth and branching by modulating microtubule-based organelle transport through its dual interactions with microtubules and the conventional kinesin motor.

The release of Acb1 protein from yeast cells requires the ESCRT-III complex to stabilize the compartment for unconventional protein secretion (CUPS).

Analysis of the Arabidopsis V-ATPase reveals convergent evolution of differential targeting as well as redundancy of the endosomal and vacuolar isoforms during vegetative development.

SPOP-dependent degradation of interferon regulatory factor 1 curtails cell-intrinsic innate immunity, thereby contributing to immune resolution.

CaRuby-Nano is a novel red-emitting and highly sensitive functionalizable calcium indicator with a wide range of applications in neuroscience and cell biology.