Soon after fertilisation, a critical portion of the embryonic genome is switched on through the actions of maternally inherited Stella, in part through controlling the activation of transposable elements.
ME31B is a general repressor of gene expression in the Drosophila early embryo, repressing translation before the maternal-to-zygotic transition and stimulating mRNA decay after activation of the zygotic genome.
Drosophila melanogaster embryos undergo a dramatic genomic transformation in the hour preceding gastrulation, as thousands of promoters and regulatory regions become biochemically distinct before they become active.
The gene Odd-paired is a late-acting regulator of zygotic gene expression, functioning coordinately with Zelda to influence chromatin accessibility and affecting genes expressed along both axes of Drosophila embryos.
The need for efficient pre-RNA splicing during early embryonic development of Drosophila indicates that the constraints imposed by the cell cycle are a force capable of driving changes in Eukaryotic gene architecture.
Following fertilization, the pioneering transcription factors GAGA factor (GAF) and Zelda are independently required to reprogram the zygotic genome of Drosophila and activate the first wave of gene expression.
In fruit flies, maternally deposited RNA-binding proteins are removed during the maternal-to-zygotic transition via a mechanism of translational upregulation of Kondo, the key E2 enzyme, at egg activation.