David G Saliba, Pablo F Céspedes-Donoso ... Michael L Dustin
TCR and CD40L microclusters are linked in synaptic ectosomes (extracellular vesicles) and released in the immunological synapse by helper T cells and induce dendritic cell maturation and cytokine production.
During cilium-generated signaling, ciliary membrane protein trafficking is unidirectional and ciliary membrane protein composition is regulated through action in the cytoplasm of the retrograde intraflagelllar transport (IFT) motor and shedding of ciliary ectosomes.
An important rice reovirus hijacks exosomes to traverse the apical plasmalemma into saliva-stored cavities in the salivary glands of insect vectors, facilitating viral horizontal transmission into rice phloem.
Manuel Alonso Y Adell, Simona M Migliano ... David Teis
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
Matthew J Shurtleff, Morayma M Temoche-Diaz ... Randy Schekman
A cell-free reaction that reconstitutes the selective sorting of a miRNA into exosomes reveals an RNA-binding protein, YBX1, as a critical sorting factor.
The combination of NSCs with NSC-derived exosomes ameliorated the injury of brain tissue including cerebral infarction, neuronal death, and glial scarring, and promoted the recovery of motor function.
Condensation of cytosolic protein YBX1 into processing bodies reveals mechanisms for efficient and selective sorting of proteins and their cognate microRNAs into exosomes.
Ines Hernandez-Perez, Javier Rubio ... María Isabel Geli
Kazrin, a protein widely expressed in vertebrates whose depletion causes defects in cell adhesion and migration, is recruited to early endosomes and promotes dynein/dynactin-dependent traffic of endocytosed cargo to the recycling endosomes.
Exosomes from cancer-associated fibroblasts enhance the "Warburg effect" in tumors and contain de novo metabolites that can contribute to the entire compendia of central carbon metabolism within cancer cells.
