A new pipeline of electron microscopy techniques reduces the time required to visualize genetically targeted neurons and their connections by two orders of magnitude.
Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.
'Cellular Electron Microscopy 500,000 images' (CEM500K) is a highly heterogeneous, information-rich, non-redundant, unlabeled EM dataset curated to pre-train DL algorithms for better model generalization on EM segmentation tasks.
New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.
The first structure of the human epithelial sodium channel provides vital insight into the assembly, stoichiometry and molecular mechanism of the ion channel central to sodium homeostasis.
Combined light and electron microscopy reveals a new function for Arp2/3-mediated actin assembly in nuclear envelope rupture, which leads to a separation of nuclear membranes and pores from the lamina.
Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
Neocortical synapses in layer 4 of the human temporal lobe neocortex were quantitatively characterized, at the subcellular level, using high-end, high-resolution electron microscopy and 3D-volume reconstructions.
