Highly efficient, precise, and scalable CRISPR/Cas9-mediated generation of endogenously tagged alleles in a vertebrate model.

CRISPR/Cas9 based genomic editing for efficient endogenous protein tagging can be achieved by targeting non-coding sequences with two guides.

The split mNeonGreen system enables fast and efficient endogenous tagging in human iPSCs and facilitates the study of proteins in human stem cells and differentiated cells by live imaging.

New technology for manipulating genes in Drosophila facilitates gene tagging and precise modification of virtually any gene.

A new gene expression tool enables precise control of protein dosage over a large range and paves the way for new genetic discoveries.

A conditional gene inactivation system that permits the tracking of protein expression, and creates fluorescently-labelled mutant cells in both developing and adult Drosophila tissues.

The postsynaptic endocytic zone is a highly organized structure composed of a diversity of endocytic proteins.

Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.

The LOVdeg tag is a versatile tool for bacterial optogenetics, offering modular, blue light-inducible protein degradation for a range of synthetic biology and metabolic engineering applications.

A small surface protein regulates gliding motility and host cell invasion by the sporozoite stage of the malaria parasite.