Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.

Cryo-EM structure and biochemical characterization of the human nuclear exosome reveals its specific properties with respect to the yeast complex, underscoring the evolutionary conservation of the RNA-channeling mechanisms and hMTR4 helicase recruitment.

An endosomal component employs a novel PAM2-like motif to recruit a key RNA-binding protein, which explains how mRNAs and associated ribosomes are attached to endosomes during coupled transport.

Plant-unique RAB5 effector 2 (PUF2) is an effector of plant-unique ARA6, which plays a key role in plant endosomal transport, integrating functions of the two plant RAB5 groups by an unprecedented mechanism.

Chlamydia hijacks membrane trafficking proteins of the human host via the cytoplasmic domain of a secreted transmembrane protein.

During cilium-generated signaling, ciliary membrane protein trafficking is unidirectional and ciliary membrane protein composition is regulated through action in the cytoplasm of the retrograde intraflagelllar transport (IFT) motor and shedding of ciliary ectosomes.

Ankyrin-B – through interactions with PI3P lipids, dynactin and RabGAP1L – functions as a critical node in the protein circuitry underlying polarized recycling of α5β1-integrin to enable haptotaxis along fibronectin gradients.

Phosphorylation of the Eps15-like protein Pan1p regulates how endocytic compartments interact with each other and with the actin cytoskeleton.

Neurons use a non-canonical secretory network to deliver select proteins to postsynaptic sites in dendrites.

The endosomal GEF Mon1-Ccz1 binds to Atg8 on autophagosomes, and recruits its substrate, the Rab7-like Ypt7, which then mediates fusion of the autophagosomes with the lysosome.