Estimating the organization of supraspinal input to motoneurons in humans is not currently possible, but a promising reverse engineering technique has been developed using large-scale supercomputing and computational neuroscience.

A deep mutational scan of the ADAR2 deaminase domain creates a map for designing tailored ADAR2 variants, and splitting the deaminase between residues 468 and 469 enables highly transcript-specific RNA editing.

Horizon scanning has been used to identify opportunities and risks presented by 20 emerging issues in the field of biological engineering.

Motif length plays a critical role in the specification of biological function within a conserved family of RNA-binding proteins.

Structure-led protein engineering can expand the effector recognition profile of plant intracellular NLR immune receptors, providing a proof-of-principle for the development of novel disease resistance mechanisms in plants.

Ire1's RNase specificity and its cleavage sites coordination shape the evolutionary specialization of the unfolded protein response.

A genome editing resource for discovering, in Drosophila, the organization of cellular structure and function by phosphoinositide signaling.

Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.

Effector target-guided engineering has developed plant NLR immune receptors that bind and recognize stealthy pathogen effector proteins, demonstrating new disease resistance profiles in planta, and offering a promising approach to protect crops against plant pathogens.

Rapid forward engineering can be accomplished using cell-free systems, as demonstrated by the implementation and characterization of novel genetic oscillators in a cell-free system and their consequent transfer to cells.