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    1. Microbiology and Infectious Disease

    Host and viral determinants of airborne transmission of SARS-CoV-2 in the Syrian hamster

    Julia R Port, Dylan H Morris ... Vincent J Munster
    An experimental animal model provides further insights into the host and viral mechanisms underlying airborne transmission of SARS-CoV-2.
    1. Computational and Systems Biology
    2. Immunology and Inflammation

    Memory CD4 T cell subsets are kinetically heterogeneous and replenished from naive T cells at high levels

    Graeme Gossel, Thea Hogan ... Andrew J Yates
    The maintenance of memory CD4 T cells in mice relies on a continual and strikingly high level of replenishment from naive precursors, and older memory T cells may resist the influx of newer ones.
    1. Cell Biology
    2. Computational and Systems Biology

    An analysis toolbox to explore mesenchymal migration heterogeneity reveals adaptive switching between distinct modes

    Hamdah Shafqat-Abbasi, Jacob M Kowalewski ... Staffan Strömblad
    “Discontinuous” and “Continuous” migration modes are divergent mesenchymal migration strategies that arise spontaneously in parallel in an equilibrium modulated by cell-matrix attachment and actomyosin contractility.
    1. Epidemiology and Global Health

    Local human movement patterns and land use impact exposure to zoonotic malaria in Malaysian Borneo

    Kimberly M Fornace, Neal Alexander ... Chris Drakeley
    Local human movement into mosquito habitats around forest edges intensifies interactions between pathogens, insects and people, increasing exposure risks to the zoonotic malaria Plasmodium knowlesi in Malaysian Borneo.
    1. Evolutionary Biology

    Measures of genetic diversification in somatic tissues at bulk and single-cell resolution

    Marius E Moeller, Nathaniel V Mon Père ... Weini Huang
    Single-cell and bulk sequencing data are combined through theoretical modeling to reveal the number of tissue-specific stem cells, mutation, and proliferation rates under sampling.
    1. Ecology

    Rarity is a more reliable indicator of land-use impacts on soil invertebrate communities than other diversity metrics

    Andrew Dopheide, Andreas Makiola ... Ian A Dickie
    Rarity metrics reveal pervasive negative impacts of agricultural land use on soil invertebrate communities, whereas widely-used richness and diversity metrics underestimate the magnitude of these impacts.
    1. Genetics and Genomics

    Single cell functional genomics reveals the importance of mitochondria in cell-to-cell phenotypic variation

    Riddhiman Dhar, Alsu M Missarova ... Lucas B Carey
    A genome-wide quantitative microscopy screen implicates mitochondria in single cell variation in proliferation and drug resistance in yeast.
    1. Microbiology and Infectious Disease

    An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

    Xiaodan Yan, Hebin Liao ... Yingying Pu
    RiboD-PETRI, a cost-effective, equipment-free plate-based scRNA-seq technique that integrates a unique bacterial rRNA depletion protocol, significantly enhancing mRNA detection rate and revealing within-population heterogeneity, offering valuable insights for research applications.
    1. Plant Biology

    Increased signal-to-noise ratios within experimental field trials by regressing spatially distributed soil properties as principal components

    Jeffrey C Berry, Mingsheng Qi ... Rebecca S Bart
    A method to reduce the noise present in field experiments by accounting for heterogeneity in field soils.
    1. Cell Biology

    Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states

    Andrew C Bergen, Rachel A Kocik ... Audrey P Gasch
    Counterintuitively, activation of the transcriptional repressor of growth-promoting genes is important for yeast cell acclimation to salt stress, such that cells with larger activation of the repressor tend to have faster growth acclimation after stress.