4,168 results found
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering

    Friso S Aalbers et al.
    By combining structure-based computational predictions and a thorough structural analysis, a highly thermostable enzyme, alcohol dehydrogenase, has been engineered.
    1. Microbiology and Infectious Disease

    Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins

    Nitzan Koppel et al.
    A unique, widely distributed gut bacterial enzyme selectively metabolizes plant-derived cardiac glycoside drugs.
    1. Computational and Systems Biology

    Design principles of autocatalytic cycles constrain enzyme kinetics and force low substrate saturation at flux branch points

    Uri Barenholz et al.
    The stability of metabolic autocatalytic cycles that are widespread in central metabolism limits the affinities of enzymes at flux branch points and explains excess expression of these enzymes.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation

    Ivana Petrovska et al.
    A starvation-induced drop in cytosolic pH promotes assembly of budding yeast glutamine synthetase into enzymatically inactive filaments that function as enzyme storage depots.
    1. Cell Biology
    2. Physics of Living Systems

    Golgi compartments enable controlled biomolecular assembly using promiscuous enzymes

    Anjali Jaiman, Mukund Thattai
    By partitioning promiscuous enzymes across multiple Golgi compartments, eukaryotic cells can tightly control the synthesis of complex sugar oligomers.
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Cryptic genetic variation shapes the adaptive evolutionary potential of enzymes

    Florian Baier et al.
    Genetic change among enzyme orthologous with similar phenotypic properties can cause substantial differences in evolutionary response to a new enzyme function in terms of their molecular and fitness outcomes.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals

    Inge Kühl et al.
    Comparative -omic analyses of five knockout mouse strains with disrupted mitochondrial DNA expression at different levels provide a high quality resource of altered gene expression patterns that reveal several common secondary patophysiological changes of mitochondrial dysfunction.
    1. Biochemistry and Chemical Biology

    Long-lived metabolic enzymes in the crystalline lens identified by pulse-labeling of mice and mass spectrometry

    Pan Liu et al.
    By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
    1. Chromosomes and Gene Expression

    Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome association and RNA:DNA hybrid formation

    Oscar Velazquez Camacho et al.
    An RNA-mediated mechanism that stabilizes association of the Suv39h enzymes at mouse heterochromatin is defined.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Relief of autoinhibition by conformational switch explains enzyme activation by a catalytically dead paralog

    Oleg A Volkov et al.
    A catalytically dead paralog activates its cognate enzyme through an allosteric mechanism that combined structural and phylogenomic analysis indicates arose through acquisition of a dimerization domain, suggesting a general model for how complex allostery evolves.

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