An integrated biochemical and evolutionary analysis shows how enzyme specificity evolves after gene loss during genome decay, implicating relaxation of purifying selection as a driving force for functional divergence.
Reconstructing ancestral enzymes has revealed that a switch in kinase substrate preference evolved via an expanded specificity intermediate that is tolerated in vivo, thus providing a path for kinase diversification.
The nerve growth-repellent activity that generates spinal nerve repeat-patterning in birds and mammals is identified at the molecular level, and a similar system is revealed in adult brain grey matter.
ZCWPW1 is a histone modification reader that localizes to DMC1-labelled double-strand break hotspots in a largely PRDM9-dependent manner, where it facilitates completion of synapsis by mediating DSB repair process.
Genetics, in vivo imaging, and unbiased chemical biology screens reveal that Trpv6 functions as a cellular quiescence regulator and delineates a Trpv6-mediated Ca2+ signaling pathway maintaining the quiescent state.