MicroRNA-based direct conversion of human fibroblasts to neurons is applicable to fibroblasts from donors ranging in age from neonatal to centenarian, allowing the generation of neurons that maintain the age-associated signatures of the starting fibroblasts.
Certain types of 3D chromatin loops are easy to predict from existing or easily obtainable 2D information, which benefits gene expression studies in tissues/cells/organisms without extensive pre-existing 3D information.
The molecular identity of bi-fated tendon-to-bone attachment cells, which display a mixture of transcriptomes of two neighboring cell types, enables the formation of the unique transitional tissue of the enthesis.
Cell lineage tracing and biochemical analysis of cell fate during murine stem cell differentiation demonstrates a specific cooperativity between perturbations in histone methylation and eroded telomeres that destabilize cell differentiation.
Doubletime kinase stabilizes the PER/TIM complex and regulates its transcriptional inhibition function to delay circadian transcriptional activity, helping sustain 24-hour periodicity in the circadian clock.