Combination of experimental mouse models with single-cell RNA-sequencing creates a detailed map of medullary thymic epithelial cell development and identifies a transit-amplifying population as the immediate precursor to Aire-expressing mTECs.
Knocking out Folliculin (FLCN) in human renal epithelial cells activates STAT1/2-mediated gene expression, independent of interferon, uncovering a tissue-specific process potentially relevant in the cancer syndrome Birt-Hogg-Dubé (BHD).
Parallel studies in primary human airway cells and stem cell-derived enteroids show that Enterovirus D-68 differentially infects and induces innate immune signaling in the respiratory and intestinal epithelium.
Time-lapse imaging and the modular recreation of host physiology reveal that alveolar epithelial cells, potential permissive infection sites for Mycobacterium tuberculosis, can restrict early bacterial growth via surfactant secretion.
Thymus ageing is characterised by both compositional and transcriptional shifts amongst epithelial cells that perturb their differentiation, contribute to organ atrophy and ultimately impair immune function.
Immunolabelling and morphological assessment, complemented by complete transcriptomic analysis, demonstrates that supporting cells can be induced to convert towards a hair cell-like phenotype in human vestibular sensory epithelia.
Epithelia exhibit size-dependent growth dynamics caused by a decoupling between boundary and bulk cellular dynamics that enable robust expansion and drive cell cycling, collective migration, and tissue-spanning vortices.