A high-throughput functional genomics approach combining inducible CRISPR-interference and quantitative imaging yields an atlas of 'phenoprints' to guide gene function assignments, identify metabolic pathway-specific morphotypes, and inform antibiotic mechanism-of-action studies.

Lin37 is essential for cell-cycle gene repression by the DREAM complex in cellular quiescence and a combined loss of Lin37 and Rb prevents cell-cycle exit in response to growth-limiting signals.

Heterochromatic sequences evolve rapidly, as do ZAD-ZNF genes-encoding proteins involved in heterochromatin functions, explaining why evolutionarily dynamic ZAD-ZNF genes are more likely to be essential in Drosophila.

When an essential metabolic gene in E. coli is mutationally inactivated, subsequent evolution rarely reverts the mutation to wild type but rather follows unexpected paths that rewire metabolic fluxes.

Large-scale developmental defects in mice occur with the ablation of the epithelial-specific splicing factors Esrp1 and Esrp2.

Following fertilization, the pioneering transcription factors GAGA factor (GAF) and Zelda are independently required to reprogram the zygotic genome of Drosophila and activate the first wave of gene expression.

The condensin I subunit Cap-G is expressed in post-mitotic neurons and its removal, especially from less mature neurons, results in gene expression changes, reduced survival and behavioural defects in Drosophila.

SRSF10 directly binds genes functioning in spermatogonia and regulates their alternative splicing, the defect of which can lead to progenitor expansion failure.

In contrast with earlier conclusions, negative selection has a major role in cancer evolution.

A near-complete flux balance analysis model of a minimal cell demonstrates the high essentiality of its metabolic genes, agrees well with experimental essentiality data and suggests some further gene removals.