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    1. Developmental Biology
    2. Evolutionary Biology

    Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms

    Yoseop Yoon, Jeff Klomp ... Urs Schmidt-Ott
    Isoform-specific transcript localization underlies the evolutionary diversity of anterior axis determinants in embryos of dipteran insects.
    1. Cell Biology
    2. Evolutionary Biology

    Lipid droplet biology and evolution illuminated by the characterization of a novel perilipin in teleost fish

    James G Granneman, Vickie A Kimler ... Ryan Thummel
    Phylogenetic, biochemical, and genetic techniques reveal a novel and ancient member of the Perilipin family, termed Plin6, that functions to concentrate and traffic lipophilic skin pigment in teleost fish.
    1. Computational and Systems Biology
    2. Neuroscience

    A connectome and analysis of the adult Drosophila central brain

    Louis K Scheffer, C Shan Xu ... Stephen M Plaza
    New reconstruction methods are used to create a publicly available dense reconstruction of the neurons and chemical synapses of central brain of Drosophila, with analysis of its graph properties.
    1. Evolutionary Biology

    Evolutionary consequences of nascent multicellular life cycles

    Jennifer T Pentz, Kathryn MacGillivray ... William C Ratcliff
    Clonal development, but not aggregation, promotes the transition of Darwinian individuality from cells to groups, emphasizing the fundamental role of initial multicellular life cycles in the evolution of multicellularity.
    1. Evolutionary Biology

    Rampant tooth loss across 200 million years of frog evolution

    Daniel J Paluh, Karina Riddell ... David C Blackburn
    Teeth are maintained in two amphibian groups (caecilians and salamanders) but were lost in frogs over 20 times independently, a higher incidence of edentulism than any other major vertebrate group.
    1. Cell Biology
    2. Developmental Biology

    Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

    Spencer R Katz, Maksim A Yakovlev ... Keith C Cheng
    Melanin staining for micro-CT imaging, using a novel application of ionic silver deposition, enables qualitative and quantitative 3D analysis of zebrafish pigmentation and reveals subtle phenotypes missed by light microscopy.
    1. Computational and Systems Biology
    2. Neuroscience

    A generalizable brain extraction net (BEN) for multimodal MRI data from rodents, nonhuman primates, and humans

    Ziqi Yu, Xiaoyang Han ... Jianfeng Feng
    The Brain Extraction Network (BEN) provides a robust, accurate, and generalizable solution not only for extracting brain tissue from multimodal MRI data in rodents, non-human primates, and humans, but also for improving the accuracy of downstream neuroimaging processing tasks.
    1. Evolutionary Biology
    2. Neuroscience

    Divergent sensory investment mirrors potential speciation via niche partitioning across Drosophila

    Ian W Keesey, Veit Grabe ... Bill S Hansson
    Phototaxis and courtship behavioral preferences reflect strong correlation with differences in olfactory and visual nervous system investment across five monophyletic Drosophila species, and could help explain their speciation events.
    1. Computational and Systems Biology
    2. Developmental Biology

    Untwisting the Caenorhabditis elegans embryo

    Ryan Patrick Christensen, Alexandra Bokinsky ... Hari Shroff
    Open-source software can untwist images of live Caenorhabditis elegans embryos, allowing epidermal and neuronal cell position and morphology to be examined in previously inaccessible developmental time periods.
    1. Developmental Biology
    2. Evolutionary Biology

    Cortex cis-regulatory switches establish scale colour identity and pattern diversity in Heliconius

    Luca Livraghi, Joseph J Hanly ... Chris D Jiggins
    The evolution of a butterfly mimetic phenotype is driven by modular cis-regulstory switches controlling a ‘hotspot’ gene.