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    1. Microbiology and Infectious Disease
    2. Plant Biology

    Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

    Derek S Lundberg, Pratchaya Pramoj Na Ayutthaya ... Detlef Weigel
    hamPCR is a cost-effective and transformative amplicon sequencing strategy to describe microbiota composition and measure its overall abundance, applicable to all samples in which host and microbial DNA are co-extracted.
    1. Neuroscience

    Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors

    Alyssa Huff, Marlusa Karlen-Amarante ... Jan-Marino Ramirez
    The postinspiratory complex acts as an interface between the swallow pattern generator and the preBötzinger complex to coordinate swallow and breathing.
    1. Neuroscience

    Neural effects of continuous theta-burst stimulation in macaque parietal neurons

    Maria C Romero, Lara Merken ... Marco Davare
    Electrophysiological recordings in awake behaving monkeys show the first evidence of the effect of continuous theta-burst stimulation, a widely used repetitive transcranial magnetic stimulation protocol, at the level of single neurons.
    1. Neuroscience

    A dynamic clamp protocol to artificially modify cell capacitance

    Paul Pfeiffer, Federico José Barreda Tomás ... Susanne Schreiber
    Via the capacitance clamp, electrophysiologists can for the first time flexibly set an artificial membrane capacitance for neurons and other excitable cells and thereby adjust their membrane time constant independent of any conductance changes.
    1. Structural Biology and Molecular Biophysics

    Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase

    Thomas-O Peulen, Carola S Hengstenberg ... Christian Herrmann
    Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Science Forum: The Brazilian Reproducibility Initiative

    Olavo B Amaral, Kleber Neves ... Clarissa FD Carneiro
    Researchers are undertaking a systematic assessment of the reproducibility of biomedical science in Brazil.
    1. Neuroscience

    Non-invasive imaging of CSF-mediated brain clearance pathways via assessment of perivascular fluid movement with diffusion tensor MRI

    Ian F Harrison, Bernard Siow ... Jack A Wells
    The first non-invasive technique to assess the action of brain clearance mechanisms, driven by the perivascular inflow of cerebrospinal fluid, has been developed using magnetic resonance imaging.
    1. Neuroscience

    Visualization of currents in neural models with similar behavior and different conductance densities

    Leandro M Alonso, Eve Marder
    Visualization methods display the dynamics of the currents in conductance-based model neurons, and show how their contribution changes in response to perturbation.
    1. Neuroscience

    Patterned perturbation of inhibition can reveal the dynamical structure of neural processing

    Sadra Sadeh, Claudia Clopath
    Neuronal networks with strong functional specificity of connections between excitatory and inhibitory neurons show specific dynamics of inhibitory stabilization, accompanied with rapid spontaneous activity transitions between highly selective functional states.
    1. Computational and Systems Biology
    2. Developmental Biology

    A comprehensive model of Drosophila epithelium reveals the role of embryo geometry and cell topology in mechanical responses

    Mohamad Ibrahim Cheikh, Joel Tchoufag ... Konstantin Doubrovinski
    Direct mechanical microcantilever-based measurements establish key material parameters describing mechanical response of the early Drosophila embryonic epithelium.