Automated segmentation of neurons and identification of synapses in electron micrographs is significantly improved by using simple modifications to chemical fixation protocols that preserve extracellular space in the brain.

Disulfide-bridged fibroblast growth factor 2 (FGF2) dimerization at the inner plasma membrane leaflet produces the building block for higher FGF2 oligomers that drive FGF2 membrane translocation into the extracellular space.

Supporting cells in the cochlea change their shape in response to purinergic receptor activation, which influences hair cell excitability by altering potassium redistribution in the extracellular space.

Solute movement in brain extracellular space is determined by solute diffusion, and does not depend on convection or aquaporin-4 expression as predicted by the glymphatic mechanism.

Snap-freezing of brain tissue reveals its true structure-showing that cells are less squashed together, and the connections between neurons are less enclosed than previously thought.

A multidisciplinary approach provides a description of the exocytic pathway that exports Salmonella Typhi's typhoid toxin from infected cells.

Different cytoneme types require specific heparan sulfate proteoglycans (HSPGs) that are stratified within the extracellular matrix in Drosophila.

Large-volume light microscopy combined with higher-resolution electron tomography revealed the spatial distribution of virus-producing cells and highlighted mechanisms of HIV-1 dissemination in bone marrow from a small animal model.

Cryo preservation of brain tissue reveals dendritic spines with thinner necks compared to those prepared with chemical fixation.

Theoretical predictions and experiments on multicellular aggregates indicate that the extracellular matrix acts as a mechanical sensor, which regulates cell proliferation and migration in a three-dimensional environment.