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    1. Neuroscience

    Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation

    Natalya Korogod et al.
    Snap-freezing of brain tissue reveals its true structure-showing that cells are less squashed together, and the connections between neurons are less enclosed than previously thought.
    1. Cell Biology
    2. Computational and Systems Biology

    A quantitative approach for analyzing the spatio-temporal distribution of 3D intracellular events in fluorescence microscopy

    Thierry Pécot et al.
    QuantEv is a fully automatic and semi-parametric method that allows quantitative analysis of the spatio-temporal distribution of complex molecular trafficking objects at the scale of the whole cell.
    1. Cell Biology
    2. Developmental Biology

    A polarity pathway for exocyst-dependent intracellular tube extension

    Joshua Abrams, Jeremy Nance
    Lumen extension in intracellular tubes relies on a molecular pathway involving Rho GTPase signaling, cell polarity, and vesicle-tethering proteins.
    1. Neuroscience

    Otoferlin acts as a Ca2+ sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses

    Nicolas Michalski et al.
    Targeted mutations in a Ca2+-binding site of otoferlin, a transmembrane protein of synaptic vesicles defective in a recessive form of deafness, reveal its Ca2+ sensor role both for vesicle fusion and vesicle pool replenishment.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles

    Maia Azubel et al.
    Specific attachment of molecularly defined gold nanoparticles enables precise localization, critical for structural studies in vivo, of proteins of unknown structure within the cellular milieu by cryo-electron tomography.
    1. Cell Biology

    Tetherin is an exosomal tether

    James R Edgar et al.
    Blocking acidification causes endosomes to fuse with the plasma membrane, releasing exosomes that remain attached to the cell surface via the anti-viral protein tetherin.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

    Javier Periz et al.
    In contrast to current knowledge that predicts that apicomplexan actin is unconventional, use of Chromobodies demonstrate that Toxoplasma F-actin forms a long, stable, highly dynamic tubular network that is required for material transfer and parasite maturation.
    1. Cell Biology
    2. Neuroscience

    A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells

    Philippe FY Vincent et al.
    A synaptic F-actin network tightly controls the flow of synaptic vesicles during exocytosis at the inner hair cell ribbons.
    1. Microbiology and Infectious Disease

    Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer

    Yang Li et al.
    L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading and transmission of leptospirosis.
    1. Neuroscience

    Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus

    Jozsef Meszaros et al.
    Dopamine release within the mouse external globus pallidus, an area of very sparse innervation, is observed and described for the first time through a new technique: flashing false fluorescent neurotransmitters.