Single-molecule localization imaging shows that the Nipah virus fusion protein forms nanoscale clusters on cell and viral membranes that favor membrane fusion activation.

By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.

Distinct frequency-based interactions between cortical and subcortical regions highlight the cerebellum’s crucial role in speech production and perception.

CTFFIND5 estimates the thickness and tilt of cryogenic electron microscopy samples directly from the power spectrum of a single exposure.

Mutagenizing sequences enriched with promoter motifs creates gene expression de novo, and helps to understand promoter logic, regulatory evolution, and the birth of de novo genes.