Long-term live imaging in intact developing Drosophila brains reveals a role for N-Cadherin-mediated fast filopodial dynamics and growth cone stabilization during neural circuit assembly.
Local inward plasma membrane deformations caused by actomyosin-dependent contraction trigger the dynamic recruitment of a curvature-sensitive actin-regulatory protein, which represents a receptor-independent auto-regulatory mechanism to control local actin polymerization dynamics at the plasma membrane.
Functional analysis of filopodia by specific interference with their formation and distribution reveals a critical role in conferring intracellular polarity and in controlling the dynamic properties of chemokine-guided cell migration in vivo.
Axonal arborisation growth is regulated by dynamic, focal localisations of Neurexin and Neuroligin that provide stability for filopodia, enabling a 'stick and grow'-based mechanism, wholly independent of synapse formation.
Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.
Asymmetric localization of the receptor EGFR within branches of axons is required to establish the precise wiring of neuronal networks within the Drosophila brain.
Direct interaction between Hedgehog-sending and Hedgehog-receiving cytonemes is a fundamental mechanism for morphogen transfer and gradient establishment.
Long-range Ret receptor signaling promotes axon outgrowth and regulates growth cone dynamics in pioneer neurons by inducing transcriptional changes in Myosin-X expression.
