4,934 results found
    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Optical estimation of absolute membrane potential using fluorescence lifetime imaging

    Julia R Lazzari-Dean et al.
    Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
    1. Developmental Biology

    In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer

    Li He et al.
    A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
    1. Cell Biology
    2. Plant Biology

    Fluorescent sensors reporting the activity of ammonium transceptors in live cells

    Roberto De Michele et al.
    A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.
    1. Physics of Living Systems

    Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

    Antonia Franziska Eckert et al.
    A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
    1. Neuroscience

    High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo

    Guanghan Meng et al.
    Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters

    Cheng-Hsun Ho, Wolf B Frommer
    Peptide and nitrate transporters have been converted into fluorescent reporters of transport activity and have been used to measure various transport properties including the dual affinity of the nitrate transceptor
    1. Structural Biology and Molecular Biophysics

    Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

    Huaying Zhao et al.
    Fluorescence detected sedimentation velocity offers a new method for studying heterogeneous protein interactions in solution by exploiting characteristic temporal signal modulations of photoswitchable fluorescent proteins.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

    Nami Kitajima et al.
    Hybrid-type fluorescent ATP sensor showing submicromolar affinity, a large fluorescence response, high selectivity and pH-independence visualized extracellular ATP dynamics in the brain of living mice with high spatiotemporal resolution.
    1. Neuroscience

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

    Richi Sakaguchi et al.
    A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    Length-dependent flagellar growth of Vibrio alginolyticus revealed by real time fluorescent imaging

    Meiting Chen et al.
    The length-dependent growth rate of bacterial flagellar filament is determined by the flagellin loading speed, loading strength and its diffusion process.

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