The mechanistic basis of molecular movement and interactions within plant root cells can be quantified using scanning fluorescence correlation spectroscopy.
A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.
Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.
Peptide and nitrate transporters have been converted into fluorescent reporters of transport activity and have been used to measure various transport properties including the dual affinity of the nitrate transceptor
Hybrid-type fluorescent ATP sensor showing submicromolar affinity, a large fluorescence response, high selectivity and pH-independence visualized extracellular ATP dynamics in the brain of living mice with high spatiotemporal resolution.
Fluorescence detected sedimentation velocity offers a new method for studying heterogeneous protein interactions in solution by exploiting characteristic temporal signal modulations of photoswitchable fluorescent proteins.
