A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.
Peptide and nitrate transporters have been converted into fluorescent reporters of transport activity and have been used to measure various transport properties including the dual affinity of the nitrate transceptor
Fluorescence detected sedimentation velocity offers a new method for studying heterogeneous protein interactions in solution by exploiting characteristic temporal signal modulations of photoswitchable fluorescent proteins.
A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
The length-dependent growth rate of bacterial flagellar filament is determined by the flagellin loading speed, loading strength and its diffusion process.
An optimized 3D fluorescence co-localization method is a useful toolkit to obtain cellular 3D separations between green and red labeled protein domains with nanometer-scale accuracy using light microscopy.
Dopamine release within the mouse external globus pallidus, an area of very sparse innervation, is observed and described for the first time through a new technique: flashing false fluorescent neurotransmitters.
