Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

The level of heterogeneity of cellular energy metabolism increases with model complexity and is the highest in patients' tumors, which can be observed and quantified using FLIM of NAD(P)H.

In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.

Heterogeneity in cytoplasmic calcium concentration alongside B cell activation and differentiation is measured intravitally using an interdisciplinary imaging approach and novel numerical analysis.

A landmark-based cross-modality alignment method robust to variation in landmark sets is applied to annotate an EM time series of Caenorhabditis elegans embryonic development as a community resource.

Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.

A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.

Mitochondrial metabolic fluxes display a subcellular spatial gradient within a single mouse oocyte, and the fluxes are not controlled by nutrient supply or energy demand of the cell, but by the intrinsic rates of mitochondrial respiration.

Simultaneous calcium imaging and cell-attached recording in GCaMP6 transgenic mice in vivo reveals spiking-fluorescence relationship that supports more refined inference of neuronal activities from calcium imaging data.

Longitudinal imaging of synapses in the brain shows that sensory deprivation differentially modifies specific synapses within individual neurons across distinct layers of the sensory cortex.