Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.

High-quality fluorescence live-cell and single molecule imaging via computer-controlled, user-assembled microscope that fits in incubator, can in large parts be 3D printed and employs open source software and electronics.

A properly designed two-photon fluorescence microscope allows high-resolution visualization of neurons and capillaries in healthy and diseased mouse retinas in vivo, while to visualize subcellular structures, adaptive optics is required to correct the eye-induced optical aberrations.

Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.

A novel single-objective light-sheet microscope with unparalleled spatiotemporal resolution enables imaging and optical manipulation of diverse biological specimens and processes.

In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.

A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.

A physics-based, statistically rigorous mathematical model enables automated, objective interpretation of images from single-molecule fluorescence colocalization microscopy experiments.

SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.

A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.