Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.
A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.
A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
QuantEv is a fully automatic and semi-parametric method that allows quantitative analysis of the spatio-temporal distribution of complex molecular trafficking objects at the scale of the whole cell.
Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.
Two optically transparent substrates enable the exploration of the ecophysiology and spatiotemporal organization and activities of bacteria and fungi within heterogeneous soil-like environments.
Asymmetric tension increase along the cell equator promotes self-organization of actomyosin into a partially aligned network during cytokinetic cleavage furrow ingression of vertebrate cells.
