3,429 results found
    1. Cell Biology
    2. Developmental Biology

    Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

    Claudia Prahst et al.
    Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.
    1. Physics of Living Systems

    Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

    Antonia Franziska Eckert et al.
    A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
    1. Structural Biology and Molecular Biophysics
    2. Immunology and Inflammation

    Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation

    Daniëlle Rianne José Verboogen et al.
    A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    3D visualization of macromolecule synthesis

    Timothy J Duerr et al.
    Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines

    Frederic Strobl et al.
    In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
    1. Ecology
    2. Microbiology and Infectious Disease

    Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms

    Kriti Sharma et al.
    Two optically transparent substrates enable the exploration of the ecophysiology and spatiotemporal organization and activities of bacteria and fungi within heterogeneous soil-like environments.
    1. Neuroscience

    Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope

    Karen J Thompson et al.
    A new method allows silver- and gold-labeled tissues to be imaged with confocal microscopy, avoiding the problem of fluorescence photobleaching.
    1. Cell Biology

    Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments

    Felix Spira et al.
    Asymmetric tension increase along the cell equator promotes self-organization of actomyosin into a partially aligned network during cytokinetic cleavage furrow ingression of vertebrate cells.
    1. Neuroscience

    Sources of widefield fluorescence from the brain

    Jack Waters
    Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.
    1. Neuroscience

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

    Richi Sakaguchi et al.
    A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.

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