The discovery of a fluorescent protein that can be rapidly switched between long-lived ‘on’ and ‘off’ states will lead to a new generation of super-resolution imaging experiments on living cells.

A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.

Late morphogenesis of the insect extraembryonic epithelia involves transient formation of a basal-basal bilayer to coordinate the functional contributions of two discrete tissues.

Building on previous work (Grotjohann et al., 2012), low-light super-resolution microscopy has been performed on living transgenic Drosophila larvae and tissues.

Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.

A novel transgenic lipid droplet reporter in transparent casper zebrafish allows direct visualization of individual lipid droplets in a living animal.

Iodixanol supplementation provides a simple method for tuning the refractive index of live imaging media, which can greatly improve resolution and penetration depth in live imaging experiments.

Quantitative super resolution imaging, in live mammalian cells, reveals a direct relationship between protein clustering dynamics and the number of mRNA transcribed at an endogenous gene locus.

A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.