The automation of cryo-focused ion beam milling enables previously unfeasible cryo-electron tomography projects.

Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.

Mature myelin sheaths are scaffolded by the anillin-dependent assembly of septin filaments, thereby facilitating rapid nerve conduction in the healthy CNS.

In situ cryo-electron tomography unveils the molecular sociology of a developing sporangium in Bacillus subtilis, revealing critical information about cell wall remodeling and membrane migration in bacteria.

The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.

The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.

Strategies to improve public trust in biomedical research are being hindered by a scientific mindset that stifles interest in reform.

Analysis of data on drug-gene interactions suggests that decentralized collaboration will increase the robustness of scientific findings in biomedical research.

The Janelia Research Campus opened its doors 12 years ago: we describe the philosophy underpinning Janelia, the lessons learned, and plans for the future.