Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
Tn5 transposase has direct tagmentation activity towards RNA/DNA hybrids, which is harnessed as a more convenient and faster RNA-seq library construction method and will benefit RNA and chromatin research.
Surrogate agonist peptide ligands discovered through screening of an IAb-peptide library potentiate expansion of visceral adipose tissue resident regulatory T cells and protect mice from inflammation and improve metabolic indices.
Single cell expression data can be used to determine how regulatory transcription factors and target genes are connected, and is especially useful when studying transcription factors controlling heterogeneous cell states.
Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.