Quantitative analyses of multi-dimensional microscopy datasets with a new Fiji/ImageJ plugin for cell tracking reveal the lineage restrictions and morphogenetic cellular behaviors underlying embryonic limb outgrowth in the direct developing crustacean Parhyale hawaiensis.
Understanding the coordination of the forces generated in embryos by two processes, convergent extension and convergent thickening, is key to understanding how a hollow sphere of cells develops into an elongated embryo.
Blastopore closure in Xenopus is driven by two morphogenic mechanisms that have strongly context dependent effects on tissue movement and that generate tensile force across tissues: convergent extension, as expected, and, unexpectedly, convergent thickening.
In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
Different developmental stages of a venomous animal (e.g. Nematostella vectensis) with a complex life cycle produce vastly different venoms that can serve in different antagonistic interactions with other species.