Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.
Surprising connections between gene architecture and splicing kinetics are illuminated using short, progressive metabolic labeling/RNA sequencing and novel computational modeling approaches in Drosophila cells.
Sox2 transcription is not correlated with spatial proximity of its essential regulatory enhancer in embryonic stem cells, suggesting gene transcription is not limited to periods of direct enhancer-promoter contact.
Combined cell labelling with a bi-cistronic reporter-gene vector and gold nanorods enables short- and long-term cell tracking in vivo via multimodal imaging (multispectral optoacoustic tomography, bioluminescence, fluorescence) with high spatial resolution.
Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.
Development and application of highly sensitive in situ transcriptomics method, Flura-seq, in identifying dynamic organ-specific transcriptomes in early stage breast cancer metastasis have been described.
Identification of two arbuscular mycorrhiza-specific lipid biosynthesis mutants and fatty acid transfer experiments reveal that host plant lipids are transferred to symbiotic fungi and needed for their development.