3,742 results found
    1. Microbiology and Infectious Disease

    Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors

    Moirangthem Kiran Singh, Parisa Zangoui ... Linda J Kenney
    Genetic code expansion (GCE) enables site-specific labeling and visualization of Salmonella secreted effectors, secretion system components and provides a viable alternative for labeling proteins that do not tolerate N- or C-terminal tags.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    3D visualization of macromolecule synthesis

    Timothy J Duerr, Ester Comellas ... James R Monaghan
    Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.
    1. Neuroscience

    Partial connectomes of labeled dopaminergic circuits reveal non-synaptic communication and axonal remodeling after exposure to cocaine

    Gregg Wildenberg, Anastasia Sorokina ... Bobby Kasthuri
    3D serial electron microscopy reveals that individual dopamine axons in the nucleus accumbens contain different varicosity types based on their vesicle contents, rarely make synapses, and undergo major structural plasticity in response to cocaine.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    A distinct transition from cell growth to physiological homeostasis in the tendon

    Mor Grinstein, Heather L Dingwall ... Jenna Lauren Galloway
    Mitotic labeling defines a change in cell division kinetics from growth to homeostasis and identifies slowly cycling cells in the adult tendon.
    1. Genetics and Genomics

    One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

    Wenyuan Li, Yage Zhang ... Bo Zhang
    Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.
    1. Genetics and Genomics
    2. Neuroscience

    A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons

    Benjamin W Okaty, Nikita Sturrock ... Susan M Dymecki
    Dorsal raphe Pet1 neurons are molecularly heterogeneous, comprising as many as fourteen distinct subtypes that show biased cell body distributions across dorsal raphe subdomains.
    1. Cancer Biology
    2. Cell Biology

    Club cells form lung adenocarcinomas and maintain the alveoli of adult mice

    Magda Spella, Ioannis Lilis ... Georgios T Stathopoulos
    Airway cells are required for the maintenance of the adult mouse lung and for carcinogen-induced lung adenocarcinoma development, and are thus marked therapeutic targets.
    1. Cell Biology

    Inclusive, exclusive and hierarchical atlas of NFATc1+/PDGFR-α+ cells in dental and periodontal mesenchyme

    Xue Yang, Chuyi Han ... Fanyuan Yu
    The inclusive, exclusive, and hierarchical atlas of NFATc1+/PDGFR-α+ cells in dental and periodontal mesenchyme is described.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

    Tin Ki Tsang, Eric A Bushong ... Mark H Ellisman
    A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
    1. Developmental Biology
    2. Neuroscience

    Transcriptional profiling of sequentially generated septal neuron fates

    Miguel Turrero García, Sarah K Stegmann ... Corey C Harwell
    A combination of single-cell RNA sequencing of the developing septum and genetic fate mapping is used to analyze the origin of septal neurons.

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