Orai1 channels activate intermittently in motile T cells, generating calcium transients, detected by a novel genetically encoded indicator and inducing pauses that favor antigen scanning.

Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.

Salsa6f is a novel, ratiometric genetically encoded Ca²⁺ indicator that combines the power of ratiometric chemical Ca²⁺ indicators with the ability, when expressed transgenically, to image cellular Ca²⁺ signals amid complex tissue environments in vivo.

Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.

Neurite arbors of VGluT3-expressing amacrine cells (VG3-ACs) process visual information locally uniformly detecting object motion while varying in contrast preferences; and in spite of extensive overlap between arbors of neighboring cells population activity in the VG3-AC plexus encodes stimulus positions with subcellular precision.

Mitochondrial energy metabolism is post-transcriptionally regulated through an interplay between long and short non-coding RNAs.

A quantitative understanding of molecular tension sensor function enables the production of unique sensors with desired mechanical properties as well as the ability to distinguish between protein force and protein deformation in mechanosensitive processes.

Gene sharing through different isoforms can precede the formation of independent genes.

The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.

An unexpected new biological function was discovered for the universally conserved cofactor lipoate, as lipoate-binding proteins proved essential for a novel wide-spread prokaryotic sulfur oxidation pathway.