The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.
A near-infrared light-stimulable optogenetic platform enables remote and wireless manipulation of calcium signaling and immune responses both in vitro and in vivo to achieve tailored function.
Aminoglycosides are a class of antibiotics that can kill Escherichia coli by building up internal voltage through disrupting the normal consumption of ATP.
Cytotoxicity associated with APOL1 renal-risk variants occurs through its plasma-membrane localization, where aberrant channel activity drives a sustained sodium and calcium influx leading to cell swelling and eventually cell death.
FIB-SEM is used to identify comprehensively and reconstruct 192 neurons and their complete connectome for glomerulus VA1v of the Drosophila antennal lobe, in particular to reconstruct its local interneurons.
The link between the activity of large populations of cortical neurons and single neuron responses is examined in primates using a new optical-genetic method.
