Ximena Steinberg, Marina A Kasimova ... Sebastian E Brauchi
Single-molecule measurement of conformational dynamics using a genetically encoded fluorescent probe suggests that the selectivity filter region of TRPV1 channels undergoes dynamic motion during agonist activation.
Moritz Armbruster, Chris G Dulla, Jeffrey S Diamond
Fluorescent glutamate indicators distort the time course of neurotransmitter diffusion and uptake by competing with transporters, an important caveat to consider when using iGluSnFR and its analogs.
Locally activatable bioluminescence (LABL) is a genetically encoded reporter that allows real time, in vivo measurement of distinct clocks in different cells and tissues in Drosophila.
Building on previous work (Entchev et al, 2015), computational analyses reveal that the choice between redundant versus synergistic encoding in a gene expression code for food abundance is controlled by cross-talk and auto-regulation among TGF-beta and serotonin pathways.
A near-infrared light-stimulable optogenetic platform enables remote and wireless manipulation of calcium signaling and immune responses both in vitro and in vivo to achieve tailored function.
Natalie Jing Ma, Colin F Hemez ... Farren J Isaacs
Cells resolve unassigned codons with near-cognate suppression, frameshifting, and ribosomal rescue mechanisms, demonstrating that unassigned codons are permissible in both natural and engineered genetic codes as barriers to horizontal gene transfer.
Travis J Morgenstern, Jinseo Park ... Henry M Colecraft
A novel engineered protein that uncouples electrical signals from downstream biological responses in excitable cells provides a unique investigative tool with potential therapeutic applications.
L Sofia Gonzalez, Austen A Fisher ... J Elliott Robinson
Dopamine release in the nucleus accumbens in mice encodes the rate and magnitude of rapid environmental luminance changes rather than visual stimulus novelty or threat intensity.
