1,847 results found
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

    Steven Lin et al.
    Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
    1. Biochemistry and Chemical Biology

    RNA-guided retargeting of Sleeping Beauty transposition in human cells

    Adrian Kovač et al.
    Fusion proteins composed of the Sleeping Beauty transposase and catalytically inactive Cas9 target transposon integration into genomic regions specified by single guide RNAs.
    1. Developmental Biology
    2. Genetics and Genomics

    A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling

    Deepti Trivedi et al.
    A genome editing resource for discovering, in Drosophila, the organization of cellular structure and function by phosphoinositide signaling.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Selection of chromosomal DNA libraries using a multiplex CRISPR system

    Owen W Ryan et al.
    An optimized CRISPR-Cas9 system enables multiplexed genome engineering for evolving biomolecules and pathways from chromosomally integrated DNA libraries.
    1. Biochemistry and Chemical Biology
    2. Genetics and Genomics

    Transposase-assisted tagmentation of RNA/DNA hybrid duplexes

    Bo Lu et al.
    Tn5 transposase has direct tagmentation activity towards RNA/DNA hybrids, which is harnessed as a more convenient and faster RNA-seq library construction method and will benefit RNA and chromatin research.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A general strategy to construct small molecule biosensors in eukaryotes

    Justin Feng et al.
    A new method for developing biosensors from ligand binding domains that have concentration-dependent and selective responses to small molecules in yeast, mammalian cells and plants.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

    Samuel T Coradetti et al.
    Using barcoded mutagenesis and a high-throughput genetic screen results in the identification of 150 genes that affect lipid accumulation in a non-model yeast system.
    1. Ecology
    2. Chromosomes and Gene Expression

    Emerging Technology: Concerning RNA-guided gene drives for the alteration of wild populations

    Kevin M Esvelt et al.
    Combining CRISPR/Cas9 genome editing with gene drives may enable scientists to reversibly edit the genomes of diverse wild populations, an advance that could help eliminate diseases, support sustainable agriculture, and control invasive species.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    High-throughput engineering of a mammalian genome reveals building principles of methylation states at CG rich regions

    Arnaud R Krebs et al.
    Large numbers of DNA sequence variants comprehensively define how transcription factor binding sites and CG density preserve mammalian promoters from repression by DNA methylation.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages

    Chwee Tat Koe et al.
    A novel complex composed of various components of a chromatin remodeling complex, a chromatin remodeling factor and a transcription factor suppresses the dedifferentiation of intermediate neural progenitors back into neuroblasts in Drosophila.

Refine your results by:

Type
Research categories