Genome-wide CRISPRi screens and proteome profiling of lysosomes link lipid dysregulation to ESCRT disruption and proteostasis defect.

Synthetic polycystin proteins self-assemble as function channels when reconstituted in lipid vesicles.

SNAP25 disease mutations within the synaptotagmin interface impair vesicle priming and cause uncontrolled spontaneous release due to enhanced SNARE:SNARE interactions, whereas a mutation within the SNARE-bundle increases the fusion energy barrier.

Disulfide-bridged fibroblast growth factor 2 (FGF2) dimerization at the inner plasma membrane leaflet produces the building block for higher FGF2 oligomers that drive FGF2 membrane translocation into the extracellular space.

An exceedingly low speed limit was estimated for a helical hairpin formation by analyzing hours-long, incessant structural transitions.

The combination of cellular cryo-electron tomography, biochemistry, and mathematical modeling provides the first integrated structural model of the Alphavirus replication organelle.

The motility of three different kinesin-dynein pairs were analyzed and found to be strikingly similar, suggesting that bidirectional transport is not regulated by motor type, but rather by scaffold, adapter, and regulatory proteins.

Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.