Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.

Comprehensive characterization of kinase-dependent phosphorylation in the human pathogen Toxoplasma gondii identifies a trafficking adaptor complex required for virulence factor secretion and infection.

Transcriptional activation domains achieve rapid, dynamic, specific interaction with Mediator through binding of an unstructured peptide to multiple hydrophobic surfaces without particular amino acid side chain interactions.

A proposal for a complete cellular/network implementation of trial-and-error motor learning in the olivo-cerebellar system.

N6-methyladenosine (m6A) reader Pho92 is directed by Paf1C to meiotic mRNAs in an m6A-dependent and independent manner and promotes CCR4-NOT-mediated mRNA decay of m6A modified transcripts contingent on translation.

Two zebrafish Keap1-paralogs are equally adept at electrophile-sensing but manifest divergent and co-regulatory electrophile-signaling behaviors.

Single-cell transcriptomics correlated with in situ gene expression analysis provide an overview of the diversity and molecular features of cell types in the squid Loligo vulgaris.

A combination of genetic and biochemical approaches suggest that yeast Rev7 promotes DNA double-strand break repair via NHEJ and inhibits homologous recombination by blocking Mre11 nuclease and Rad50’s ATPase activities.

SNAP25 disease mutations within the synaptotagmin interface impair vesicle priming and cause uncontrolled spontaneous release due to enhanced SNARE:SNARE interactions, whereas a mutation within the SNARE-bundle increases the fusion energy barrier.

Recent findings about the possible intercellular signalling role of glutamate, GABA, and melatonin might help to establish hypotheses concerning the evolutionary factors that caused intercellular organisms to use specific molecules in intercellular signalling.