Single-cell sequencing and functional analysis identify T cells that can be useful for marker selection and cell therapy in uveal melanoma, a disease largely unresponsive to conventional immune checkpoint therapies.
Comparative -omic analyses of five knockout mouse strains with disrupted mitochondrial DNA expression at different levels provide a high quality resource of altered gene expression patterns that reveal several common secondary patophysiological changes of mitochondrial dysfunction.
Gabriel Demo, Aviram Rasouly ... Andrei A Korostelev
Cryo-EM structures of the 30S*RNAP complex visualize co-localization of the transcription and translation machineries and provide insights into the transcription-translation synchrony, which coordinates gene expression in bacteria.
Single-cell transcriptomics of the zebrafish ovary identifies both major and minor cell types and provides a valuable resource for researchers interested in reproductive biology, stem cell biology, and aquatic toxicology.
Olga Martzoukou, Sotiris Amillis ... George Diallinas
In filamentous fungi the AP-2 complex, which in mammals is an adaptor of clathrin-mediated endocytosis, is recruited to specific clathrin-independent apical endocytosis necessary for proper lipid maintenance and polar growth.
CDPK4 is a pleiotropic regulator controlling initiation of DNA replication, mitotic spindle assembly and flagellar activation during the early stages of Plasmodium transmission.
Charlotte Lässig, Sarah Matheisl ... Karl-Peter Hopfner
Mutations within the ATPase domain of RIG-I in patients with Singleton-Merten Syndrome prevent ATP-hydrolysis dependent dissociation of RIG-I from double-stranded RNA and lead to unintentional constitutive signaling through increased binding of endogenous RNA.
A gradient of Mib1-Dll4 within multinucleated muscle fibers maintains a continuum of metastable states within the muscle stem cell pool during tissue homeostasis.
