Live imaging coupled with cell lineage tracing in chick and mouse embryos reveal that the cardiac regulatory gene Nkx2.5 is also transiently expressed in early extra-cardiac hemogenic angioblasts that migrate to the heart, yolk sac and dorsal aorta.
Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.
Emergence of hematopoietic stem cells from an aortic component is a unique phenomenon of cell extrusion, with no equivalent in cell biology, and which is adapted to environmental constraints exerted by endothelial cells and hemodynamics forces.
At gastrulation, mesoderm arises as a migratory germ layer that will participate to both foetal and placental development through region-dependant adaptation of cytoskeleton composition, cell shape and migration mode.
The conserved mechanism of Notch down-regulation by endo-lysosomal trafficking plays important roles in the proliferation and differentiation of stem and progenitor cells during embryogenesis or organogenesis in vertebrates.