Oriented hexasomes can be generated using the Widom 601 positioning sequence, which enables straightforward production of nucleosomes with asymmetrically modified H2A/H2B dimers.
Cryo-EM structure and biochemical characterization of the human nuclear exosome reveals its specific properties with respect to the yeast complex, underscoring the evolutionary conservation of the RNA-channeling mechanisms and hMTR4 helicase recruitment.
The nuclear exosome cofactors Mpp6 and Rrp47 can stimulate exoribonuclease activities of the nuclear RNA exosome and recruit the Mtr4 helicase to promote helicase dependent RNA decay.
Contrary to the generally accepted model, condensin maintains proper gene expression by promoting the accurate segregation of chromosomes and the partitioning of the RNA-exosome throughout mitosis, instead of directly regulating transcription.
A stable tetrameric nucleosome occupies the central segment of each ∼120-bp budding yeast centromere in two rotational phases of both reflectional orientations in vivo.
A screen using artificially barcoded, exosomal microRNAs, paired with CRISPR guide RNAs, helped identify new players in multivesicular endosome exocytosis and a role for Wnt signaling.
A cell-free reaction that reconstitutes the selective sorting of a miRNA into exosomes reveals an RNA-binding protein, YBX1, as a critical sorting factor.
An enrichable cross-linker with optional isotope labeling quadruples the number of cross-linked peptides identified from high-complexity samples, enhancing proteome-wide analysis of protein-protein interactions and protein conformational changes by mass spectrometry.
