Zeno SWATH MS, a novel data-independent acquisition technique in proteomics, which by facilitating sensitive detection in high-throughput experiments with low sample amounts, mitigates current bottlenecks of high-throughput proteomics.
The 'low water' agar assay provides a reliable and practical high-throughput method for studying plant molecular signaling responses to drought stress.
Flavio H Beraldo, Daniel Palmer ... Marco AM Prado
Comprehensive dataset of high-level cognitive assessment in mouse models of neurodegeneration, accompanied by an open-access database/repository to change the paradigm of how cognitive studies in animal models can be shared/re-used.
Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.
David S White, Marcel P Goldschen-Ohm ... Baron Chanda
A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.
anTraX is an algorithm and software package that facilitates automated analyses of insect social behavior in species and experimental settings that are not accessible with currently existing technology.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
David Trombley McSwiggen, Helen Liu ... Hilary P Beck
Single-molecule tracking at scale, analyzing millions of cells and thousands of compounds per day, demonstrates that measuring protein motion provides mechanistic insights relevant to drug discovery.
Guangliang Wang, Surendra K Rajpurohit ... Jeff S Mumm
A novel high-throughput, whole organism chemical screening platform was used to identify existing drugs that increase pancreatic beta-cell mass in zebrafish, implicating unique roles for the NF-κB and serotonergic signaling pathways in regulating pancreatic biology.