A novel high-throughput, whole organism chemical screening platform was used to identify existing drugs that increase pancreatic beta-cell mass in zebrafish, implicating unique roles for the NF-κB and serotonergic signaling pathways in regulating pancreatic biology.
Activity-regulated genes in Drosophila neurons differ from the well-characterized situation in mammals, and these genes provided a strategy to construct reporters for monitoring neuronal activity in fly brains.
CRISPR/Cas9 gene-editing was used to generate a novel endogenous pH-sensitive EGF receptor chimera with the unique utility to study receptor endocytosis in a single-cell and high-throughput experimental formats.
Yeast promoters can harbor multiple natural DNA variants that influence gene expression, interact genetically, evolve under negative selection, alter transcription factor motifs, and remain challenging to predict.
The structure-based design established a new approach to control pathway-selective activation of opioid receptors, resulting in new dual MOR/KOR G-protein biased agonist analgesics with attenuated liabilities.